mouse anti nestin Search Results


mouse anti nestin  (R&D Systems)
99
R&D Systems mouse anti nestin
Mouse Anti Nestin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nestin reafinity  (Miltenyi Biotec)
94
Miltenyi Biotec nestin reafinity
Characteristics of <t>nestin-expressing</t> cells in the lung. A Flowcytometric analysis of nestin-expressing cells in a nestin-GFP murine lung and CD45 − lung cells. B The frequency of CD45 − /nestin-expressing cells in 9–12-week-old male and female nestin-GFP murine lungs (n = 4–8 mice per group). C (left) Representative image of nestin-expressing cells in CD45 − lung cells obtained from a 2-week-old nestin-GFP mouse. Effect of aging on (middle) frequency of CD45 − /nestin-expressing cells and (right) Nes (nestin) mRNA expression (n = 4–8 mice per group). D Representative images of cell surface markers expressed on CD45 − /nestin-expressing cells. E Fluorescent microscopic images of a nestin-GFP murine lung. Nuclei are shown in purple-blue with DAPI. Scale bars = 50 μm. F GFP immunostaining of wildtype (WT) and nestin-GFP murine lungs. Nestin signals are shown in brown with anti-GFP antibody. Scale bars = 100 μm. G Expression of Nes mRNA between CD31 + endothelial cells and CD31 − non-endothelial cells obtained from murine lungs (n = 4 per group)
Nestin Reafinity, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
nestin reafinity - by Bioz Stars, 2026-03
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monoclonal mouse anti nestin  (Bio-Rad)
93
Bio-Rad monoclonal mouse anti nestin
Characteristics of <t>nestin-expressing</t> cells in the lung. A Flowcytometric analysis of nestin-expressing cells in a nestin-GFP murine lung and CD45 − lung cells. B The frequency of CD45 − /nestin-expressing cells in 9–12-week-old male and female nestin-GFP murine lungs (n = 4–8 mice per group). C (left) Representative image of nestin-expressing cells in CD45 − lung cells obtained from a 2-week-old nestin-GFP mouse. Effect of aging on (middle) frequency of CD45 − /nestin-expressing cells and (right) Nes (nestin) mRNA expression (n = 4–8 mice per group). D Representative images of cell surface markers expressed on CD45 − /nestin-expressing cells. E Fluorescent microscopic images of a nestin-GFP murine lung. Nuclei are shown in purple-blue with DAPI. Scale bars = 50 μm. F GFP immunostaining of wildtype (WT) and nestin-GFP murine lungs. Nestin signals are shown in brown with anti-GFP antibody. Scale bars = 100 μm. G Expression of Nes mRNA between CD31 + endothelial cells and CD31 − non-endothelial cells obtained from murine lungs (n = 4 per group)
Monoclonal Mouse Anti Nestin, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse anti nestin antibody  (R&D Systems)
93
R&D Systems mouse anti nestin antibody
Characteristics of <t>nestin-expressing</t> cells in the lung. A Flowcytometric analysis of nestin-expressing cells in a nestin-GFP murine lung and CD45 − lung cells. B The frequency of CD45 − /nestin-expressing cells in 9–12-week-old male and female nestin-GFP murine lungs (n = 4–8 mice per group). C (left) Representative image of nestin-expressing cells in CD45 − lung cells obtained from a 2-week-old nestin-GFP mouse. Effect of aging on (middle) frequency of CD45 − /nestin-expressing cells and (right) Nes (nestin) mRNA expression (n = 4–8 mice per group). D Representative images of cell surface markers expressed on CD45 − /nestin-expressing cells. E Fluorescent microscopic images of a nestin-GFP murine lung. Nuclei are shown in purple-blue with DAPI. Scale bars = 50 μm. F GFP immunostaining of wildtype (WT) and nestin-GFP murine lungs. Nestin signals are shown in brown with anti-GFP antibody. Scale bars = 100 μm. G Expression of Nes mRNA between CD31 + endothelial cells and CD31 − non-endothelial cells obtained from murine lungs (n = 4 per group)
Mouse Anti Nestin Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti nestin antibody/product/R&D Systems
Average 93 stars, based on 1 article reviews
mouse anti nestin antibody - by Bioz Stars, 2026-03
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mouse monoclonal  (R&D Systems)
91
R&D Systems mouse monoclonal
Characteristics of <t>nestin-expressing</t> cells in the lung. A Flowcytometric analysis of nestin-expressing cells in a nestin-GFP murine lung and CD45 − lung cells. B The frequency of CD45 − /nestin-expressing cells in 9–12-week-old male and female nestin-GFP murine lungs (n = 4–8 mice per group). C (left) Representative image of nestin-expressing cells in CD45 − lung cells obtained from a 2-week-old nestin-GFP mouse. Effect of aging on (middle) frequency of CD45 − /nestin-expressing cells and (right) Nes (nestin) mRNA expression (n = 4–8 mice per group). D Representative images of cell surface markers expressed on CD45 − /nestin-expressing cells. E Fluorescent microscopic images of a nestin-GFP murine lung. Nuclei are shown in purple-blue with DAPI. Scale bars = 50 μm. F GFP immunostaining of wildtype (WT) and nestin-GFP murine lungs. Nestin signals are shown in brown with anti-GFP antibody. Scale bars = 100 μm. G Expression of Nes mRNA between CD31 + endothelial cells and CD31 − non-endothelial cells obtained from murine lungs (n = 4 per group)
Mouse Monoclonal, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse anti nestin  (Neuromics)
93
Neuromics mouse anti nestin
Characteristics of <t>nestin-expressing</t> cells in the lung. A Flowcytometric analysis of nestin-expressing cells in a nestin-GFP murine lung and CD45 − lung cells. B The frequency of CD45 − /nestin-expressing cells in 9–12-week-old male and female nestin-GFP murine lungs (n = 4–8 mice per group). C (left) Representative image of nestin-expressing cells in CD45 − lung cells obtained from a 2-week-old nestin-GFP mouse. Effect of aging on (middle) frequency of CD45 − /nestin-expressing cells and (right) Nes (nestin) mRNA expression (n = 4–8 mice per group). D Representative images of cell surface markers expressed on CD45 − /nestin-expressing cells. E Fluorescent microscopic images of a nestin-GFP murine lung. Nuclei are shown in purple-blue with DAPI. Scale bars = 50 μm. F GFP immunostaining of wildtype (WT) and nestin-GFP murine lungs. Nestin signals are shown in brown with anti-GFP antibody. Scale bars = 100 μm. G Expression of Nes mRNA between CD31 + endothelial cells and CD31 − non-endothelial cells obtained from murine lungs (n = 4 per group)
Mouse Anti Nestin, supplied by Neuromics, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nestin  (Bio-Rad)
92
Bio-Rad nestin
Figure 1. <t>Nestin</t> expression (red arrows) in blood vessels in NBTLs [(A) x300], DCIS [(B) x300] and IDC [(C) x300]. Nestin expression in myoepithelial cells is also observed [(A and B) green arrows]. Nestin [(D) x250], CD34 [(E) x250] <t>and</t> <t>CD31</t> [(F) x250)] expression in blood vessels (red arrows, serial sections).
Nestin, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nestin - by Bioz Stars, 2026-03
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mouse monoclonal anti nestin phycoerythrin  (R&D Systems)
92
R&D Systems mouse monoclonal anti nestin phycoerythrin
Figure 1. <t>Nestin</t> expression (red arrows) in blood vessels in NBTLs [(A) x300], DCIS [(B) x300] and IDC [(C) x300]. Nestin expression in myoepithelial cells is also observed [(A and B) green arrows]. Nestin [(D) x250], CD34 [(E) x250] <t>and</t> <t>CD31</t> [(F) x250)] expression in blood vessels (red arrows, serial sections).
Mouse Monoclonal Anti Nestin Phycoerythrin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse anti-nestin antibody  (Becton Dickinson)
90
Becton Dickinson mouse anti-nestin antibody
Figure 1. <t>Nestin</t> expression (red arrows) in blood vessels in NBTLs [(A) x300], DCIS [(B) x300] and IDC [(C) x300]. Nestin expression in myoepithelial cells is also observed [(A and B) green arrows]. Nestin [(D) x250], CD34 [(E) x250] <t>and</t> <t>CD31</t> [(F) x250)] expression in blood vessels (red arrows, serial sections).
Mouse Anti Nestin Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
mouse anti-nestin antibody - by Bioz Stars, 2026-03
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mouse anti-nestin  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc mouse anti-nestin
Figure 1. <t>Nestin</t> expression (red arrows) in blood vessels in NBTLs [(A) x300], DCIS [(B) x300] and IDC [(C) x300]. Nestin expression in myoepithelial cells is also observed [(A and B) green arrows]. Nestin [(D) x250], CD34 [(E) x250] <t>and</t> <t>CD31</t> [(F) x250)] expression in blood vessels (red arrows, serial sections).
Mouse Anti Nestin, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti-nestin/product/STEMCELL Technologies Inc
Average 90 stars, based on 1 article reviews
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nestin  (Merck KGaA)
90
Merck KGaA nestin
SSCs (p2-3) expressed stem cell markers and lacked neural crest markers. ( A ) SSCs derived from female donor 2 <t>expressed</t> <t>CD164,</t> <t>Nestin,</t> CD133, and SOX9 and lacked the expression of SLUG and p75. ( B ) Male donor 14-derived SSCs expressed CD164, Nestin, CD133, and SOX9 but did not express SLUG or p75. ( C ) Semiquantitative analysis showed high Nestin (65.6–89.4%), CD133 (43.5–96.3%), CD164 (82.7–91.0%), and SOX9 (98.1–99.3%) expression in three female SSC populations but lacking p75 (0.0–0.0%) and SLUG (1.1–2.5%) expression. ( D ) Semiquantitative analysis of three male SSC populations unraveled high levels of Nestin (55.4–88.2%), CD133 (50.9–78.8%), CD164 (62.8–75.8%), and SOX9 (97.1–99.0%), but p75 (0.0–0.0%) and SLUG (1.1–3.2%) expressions were deficient.
Nestin, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Characteristics of nestin-expressing cells in the lung. A Flowcytometric analysis of nestin-expressing cells in a nestin-GFP murine lung and CD45 − lung cells. B The frequency of CD45 − /nestin-expressing cells in 9–12-week-old male and female nestin-GFP murine lungs (n = 4–8 mice per group). C (left) Representative image of nestin-expressing cells in CD45 − lung cells obtained from a 2-week-old nestin-GFP mouse. Effect of aging on (middle) frequency of CD45 − /nestin-expressing cells and (right) Nes (nestin) mRNA expression (n = 4–8 mice per group). D Representative images of cell surface markers expressed on CD45 − /nestin-expressing cells. E Fluorescent microscopic images of a nestin-GFP murine lung. Nuclei are shown in purple-blue with DAPI. Scale bars = 50 μm. F GFP immunostaining of wildtype (WT) and nestin-GFP murine lungs. Nestin signals are shown in brown with anti-GFP antibody. Scale bars = 100 μm. G Expression of Nes mRNA between CD31 + endothelial cells and CD31 − non-endothelial cells obtained from murine lungs (n = 4 per group)

Journal: Cell Communication and Signaling : CCS

Article Title: Nestin and Notch3 collaboratively regulate angiogenesis, collagen production, and endothelial–mesenchymal transition in lung endothelial cells

doi: 10.1186/s12964-023-01099-z

Figure Lengend Snippet: Characteristics of nestin-expressing cells in the lung. A Flowcytometric analysis of nestin-expressing cells in a nestin-GFP murine lung and CD45 − lung cells. B The frequency of CD45 − /nestin-expressing cells in 9–12-week-old male and female nestin-GFP murine lungs (n = 4–8 mice per group). C (left) Representative image of nestin-expressing cells in CD45 − lung cells obtained from a 2-week-old nestin-GFP mouse. Effect of aging on (middle) frequency of CD45 − /nestin-expressing cells and (right) Nes (nestin) mRNA expression (n = 4–8 mice per group). D Representative images of cell surface markers expressed on CD45 − /nestin-expressing cells. E Fluorescent microscopic images of a nestin-GFP murine lung. Nuclei are shown in purple-blue with DAPI. Scale bars = 50 μm. F GFP immunostaining of wildtype (WT) and nestin-GFP murine lungs. Nestin signals are shown in brown with anti-GFP antibody. Scale bars = 100 μm. G Expression of Nes mRNA between CD31 + endothelial cells and CD31 − non-endothelial cells obtained from murine lungs (n = 4 per group)

Article Snippet: The antibodies used by flowcytometry and cell sorting were: CD45-PB (clone 30-F11, BioLegend), EpCAM-PE/Cy7 (clone G8.8, BioLegend), CD140a (PDGFRα)-APC (clone APA-5, BioLegend), CD140b (PDGFRβ)-APC (clone ABP5, Miltenyi Biotec, Bergisch Gladbach, Germany), CD31-APC (clone MEC13-3, BioLegend), CD31-PE/Cy7 (clone 390, BioLegend), VEGFR2-APC (clone Avas12, BioLegend), CD13-PE (clone R3-242, BD Biosciences, San Jose, CA, USA), Notch3-Alexa Fluor 647 (clone HMN3-133, BioLegend), αSMA-eFluor660 (clone 1A4, Thermo Fisher Scientific), mouse IgG2a κ Isotype Control-eFluor 660 (clone eBM2a, Thermo Fisher Scientific), Collagen Type I-FITC (600–401-103–0.1, Rockland Immunochemicals, Pottstown, PA, USA), rabbit IgG Isotype Control-FITC (bs-0295P-FITC, Bioss Antibodies, Woburn, MA, USA), CD105 (TGFβR)-APC (clone MJ7/18, BioLegend), nestin-Alexa Fluor 647 (clone Rat-401, shown as antibody A, BioLegend), Alexa Fluor 647 mouse IgG1, κ Isotype Control (clone MOPC-21, BioLegend), nestin-REAfinity (clone REA575, shown as antibody B, Miltenyi Biotec), REA Control Antibody (I), human IgG1, and REAfinity (clone REA293, Miltenyi Biotec).

Techniques: Expressing, Immunostaining

Roles of nestin-expressing endothelial cells on angiogenesis. A and B Flowcytometric analysis of ( A ) VEGFR2 or ( B ) PDGFRβ expression in nestin-expressing and -nonexpressing endothelial cells of a murine lung (n = 5 per group). C (left) Workflow of 3-dimensional (3D) culture to examine the angiogenetic ability in nestin-expressing (Nes + ) and -nonexpressing (Nes − ) endothelial cells (EC) obtained from nestin-GFP murine lungs. (middle) Obtained Nes + and Nes − ECs were cultured under 3D culture system supplemented with 50 ng/mL of VEGF or 50 ng/mL of PDGF. Representative results of 3D culture after 21 d. Scale bars = 100 μm. (right) Calculation of the vascular densities using imaging software as described in Method (n = 3 per group). D Nes mRNA expression in each murine cell line: 3T3 (fibroblasts), LA-4 (epithelial cells), and MS1 (endothelial cells) (n = 5 per group). E Effect of gene silencing using nestin siRNA on MS1 (n = 5 per group). F MS1 cells were cultured under 3D culture system supplemented with 50 ng/mL of VEGF or 50 ng/mL of PDGF. Representative results of MS1 treated with control or nestin siRNA after 6 d of 3D culture. Scale bars = 100 μm

Journal: Cell Communication and Signaling : CCS

Article Title: Nestin and Notch3 collaboratively regulate angiogenesis, collagen production, and endothelial–mesenchymal transition in lung endothelial cells

doi: 10.1186/s12964-023-01099-z

Figure Lengend Snippet: Roles of nestin-expressing endothelial cells on angiogenesis. A and B Flowcytometric analysis of ( A ) VEGFR2 or ( B ) PDGFRβ expression in nestin-expressing and -nonexpressing endothelial cells of a murine lung (n = 5 per group). C (left) Workflow of 3-dimensional (3D) culture to examine the angiogenetic ability in nestin-expressing (Nes + ) and -nonexpressing (Nes − ) endothelial cells (EC) obtained from nestin-GFP murine lungs. (middle) Obtained Nes + and Nes − ECs were cultured under 3D culture system supplemented with 50 ng/mL of VEGF or 50 ng/mL of PDGF. Representative results of 3D culture after 21 d. Scale bars = 100 μm. (right) Calculation of the vascular densities using imaging software as described in Method (n = 3 per group). D Nes mRNA expression in each murine cell line: 3T3 (fibroblasts), LA-4 (epithelial cells), and MS1 (endothelial cells) (n = 5 per group). E Effect of gene silencing using nestin siRNA on MS1 (n = 5 per group). F MS1 cells were cultured under 3D culture system supplemented with 50 ng/mL of VEGF or 50 ng/mL of PDGF. Representative results of MS1 treated with control or nestin siRNA after 6 d of 3D culture. Scale bars = 100 μm

Article Snippet: The antibodies used by flowcytometry and cell sorting were: CD45-PB (clone 30-F11, BioLegend), EpCAM-PE/Cy7 (clone G8.8, BioLegend), CD140a (PDGFRα)-APC (clone APA-5, BioLegend), CD140b (PDGFRβ)-APC (clone ABP5, Miltenyi Biotec, Bergisch Gladbach, Germany), CD31-APC (clone MEC13-3, BioLegend), CD31-PE/Cy7 (clone 390, BioLegend), VEGFR2-APC (clone Avas12, BioLegend), CD13-PE (clone R3-242, BD Biosciences, San Jose, CA, USA), Notch3-Alexa Fluor 647 (clone HMN3-133, BioLegend), αSMA-eFluor660 (clone 1A4, Thermo Fisher Scientific), mouse IgG2a κ Isotype Control-eFluor 660 (clone eBM2a, Thermo Fisher Scientific), Collagen Type I-FITC (600–401-103–0.1, Rockland Immunochemicals, Pottstown, PA, USA), rabbit IgG Isotype Control-FITC (bs-0295P-FITC, Bioss Antibodies, Woburn, MA, USA), CD105 (TGFβR)-APC (clone MJ7/18, BioLegend), nestin-Alexa Fluor 647 (clone Rat-401, shown as antibody A, BioLegend), Alexa Fluor 647 mouse IgG1, κ Isotype Control (clone MOPC-21, BioLegend), nestin-REAfinity (clone REA575, shown as antibody B, Miltenyi Biotec), REA Control Antibody (I), human IgG1, and REAfinity (clone REA293, Miltenyi Biotec).

Techniques: Expressing, Cell Culture, Imaging, Software, Control

Roles of nestin-expressing endothelial cells during EndoMT. A Morphological changes of MS1 supplemented with TGFβ in 2D culture (at 24 or 72 h), and 3D culture (at day 8). Scale bars = 100 μm. B Expression of mesenchymal-related ( Col1a1 , collagen type 1; Fn1 , fibronectin; and Acta2 , αSMA), EndoMT-related ( Snai1 , snail1 and Snai2 , snail2), and Nes mRNA in MS1 incubated for 10 h supplemented with or without TGFβ (n = 5 per group). C Expression of Nes and mesenchymal-related ( Col1a1 , Fn1 , and Acta2 ) mRNA in MS1 at 5 h of incubation with or without TGFβ (n = 5–8 per group). D Effect of nestin gene silencing of normally cultured MS1 cells on Nes , Col1a1 and Fn1 mRNA expressions (n = 5–8 per group). E Effect of nestin gene silencing in MS1 undergoing TGFβ-induced EndoMT on Nes , mesenchymal-related ( Col1a1 , Fn1 , and Acta2 ), and EndoMT-related ( Snai1 and Snai2 ) mRNA expression (n = 8 per group). To induce EndoMT, MS1 was pre-treated with TGFβ for 10 h

Journal: Cell Communication and Signaling : CCS

Article Title: Nestin and Notch3 collaboratively regulate angiogenesis, collagen production, and endothelial–mesenchymal transition in lung endothelial cells

doi: 10.1186/s12964-023-01099-z

Figure Lengend Snippet: Roles of nestin-expressing endothelial cells during EndoMT. A Morphological changes of MS1 supplemented with TGFβ in 2D culture (at 24 or 72 h), and 3D culture (at day 8). Scale bars = 100 μm. B Expression of mesenchymal-related ( Col1a1 , collagen type 1; Fn1 , fibronectin; and Acta2 , αSMA), EndoMT-related ( Snai1 , snail1 and Snai2 , snail2), and Nes mRNA in MS1 incubated for 10 h supplemented with or without TGFβ (n = 5 per group). C Expression of Nes and mesenchymal-related ( Col1a1 , Fn1 , and Acta2 ) mRNA in MS1 at 5 h of incubation with or without TGFβ (n = 5–8 per group). D Effect of nestin gene silencing of normally cultured MS1 cells on Nes , Col1a1 and Fn1 mRNA expressions (n = 5–8 per group). E Effect of nestin gene silencing in MS1 undergoing TGFβ-induced EndoMT on Nes , mesenchymal-related ( Col1a1 , Fn1 , and Acta2 ), and EndoMT-related ( Snai1 and Snai2 ) mRNA expression (n = 8 per group). To induce EndoMT, MS1 was pre-treated with TGFβ for 10 h

Article Snippet: The antibodies used by flowcytometry and cell sorting were: CD45-PB (clone 30-F11, BioLegend), EpCAM-PE/Cy7 (clone G8.8, BioLegend), CD140a (PDGFRα)-APC (clone APA-5, BioLegend), CD140b (PDGFRβ)-APC (clone ABP5, Miltenyi Biotec, Bergisch Gladbach, Germany), CD31-APC (clone MEC13-3, BioLegend), CD31-PE/Cy7 (clone 390, BioLegend), VEGFR2-APC (clone Avas12, BioLegend), CD13-PE (clone R3-242, BD Biosciences, San Jose, CA, USA), Notch3-Alexa Fluor 647 (clone HMN3-133, BioLegend), αSMA-eFluor660 (clone 1A4, Thermo Fisher Scientific), mouse IgG2a κ Isotype Control-eFluor 660 (clone eBM2a, Thermo Fisher Scientific), Collagen Type I-FITC (600–401-103–0.1, Rockland Immunochemicals, Pottstown, PA, USA), rabbit IgG Isotype Control-FITC (bs-0295P-FITC, Bioss Antibodies, Woburn, MA, USA), CD105 (TGFβR)-APC (clone MJ7/18, BioLegend), nestin-Alexa Fluor 647 (clone Rat-401, shown as antibody A, BioLegend), Alexa Fluor 647 mouse IgG1, κ Isotype Control (clone MOPC-21, BioLegend), nestin-REAfinity (clone REA575, shown as antibody B, Miltenyi Biotec), REA Control Antibody (I), human IgG1, and REAfinity (clone REA293, Miltenyi Biotec).

Techniques: Expressing, Incubation, Cell Culture

Notch3 expression is regulated by nestin in vitro. A Hierarchical clustered heatmap showing the gene expression patterns (fold change < − 2.0 or ≥ 2.0) in nestin-expressing (Nes + ) and -nonexpressing (Nes − ) endothelial cells (EC, n = 2 per group). Each row and line represent one sample and differentially expressed genes, respectively. Red and blue indicate upregulation and downregulation, respectively. B Dot plot showing the absolute value of fold change of differentially expressed genes between Nes + and Nes − EC. Notch3 is presented at sixth place. C Pericyte markers (Notch3 and CD13) expressed on CD45 − /nestin-expressing and -nonexpressing cells (n = 4 per group). D and E Effect of nestin ( D , n = 8 per group) or Notch3 ( E , n = 6 per group)-gene silencing in MS1 cells on mRNA expression. F Morphological changes in MS1 cells treated with control or Notch3 siRNA in 2D culture at 48 h. Scale bars = 100 μm

Journal: Cell Communication and Signaling : CCS

Article Title: Nestin and Notch3 collaboratively regulate angiogenesis, collagen production, and endothelial–mesenchymal transition in lung endothelial cells

doi: 10.1186/s12964-023-01099-z

Figure Lengend Snippet: Notch3 expression is regulated by nestin in vitro. A Hierarchical clustered heatmap showing the gene expression patterns (fold change < − 2.0 or ≥ 2.0) in nestin-expressing (Nes + ) and -nonexpressing (Nes − ) endothelial cells (EC, n = 2 per group). Each row and line represent one sample and differentially expressed genes, respectively. Red and blue indicate upregulation and downregulation, respectively. B Dot plot showing the absolute value of fold change of differentially expressed genes between Nes + and Nes − EC. Notch3 is presented at sixth place. C Pericyte markers (Notch3 and CD13) expressed on CD45 − /nestin-expressing and -nonexpressing cells (n = 4 per group). D and E Effect of nestin ( D , n = 8 per group) or Notch3 ( E , n = 6 per group)-gene silencing in MS1 cells on mRNA expression. F Morphological changes in MS1 cells treated with control or Notch3 siRNA in 2D culture at 48 h. Scale bars = 100 μm

Article Snippet: The antibodies used by flowcytometry and cell sorting were: CD45-PB (clone 30-F11, BioLegend), EpCAM-PE/Cy7 (clone G8.8, BioLegend), CD140a (PDGFRα)-APC (clone APA-5, BioLegend), CD140b (PDGFRβ)-APC (clone ABP5, Miltenyi Biotec, Bergisch Gladbach, Germany), CD31-APC (clone MEC13-3, BioLegend), CD31-PE/Cy7 (clone 390, BioLegend), VEGFR2-APC (clone Avas12, BioLegend), CD13-PE (clone R3-242, BD Biosciences, San Jose, CA, USA), Notch3-Alexa Fluor 647 (clone HMN3-133, BioLegend), αSMA-eFluor660 (clone 1A4, Thermo Fisher Scientific), mouse IgG2a κ Isotype Control-eFluor 660 (clone eBM2a, Thermo Fisher Scientific), Collagen Type I-FITC (600–401-103–0.1, Rockland Immunochemicals, Pottstown, PA, USA), rabbit IgG Isotype Control-FITC (bs-0295P-FITC, Bioss Antibodies, Woburn, MA, USA), CD105 (TGFβR)-APC (clone MJ7/18, BioLegend), nestin-Alexa Fluor 647 (clone Rat-401, shown as antibody A, BioLegend), Alexa Fluor 647 mouse IgG1, κ Isotype Control (clone MOPC-21, BioLegend), nestin-REAfinity (clone REA575, shown as antibody B, Miltenyi Biotec), REA Control Antibody (I), human IgG1, and REAfinity (clone REA293, Miltenyi Biotec).

Techniques: Expressing, In Vitro, Gene Expression, Control

Effect of nestin gene silencing in vivo. A (left) GFP immunostaining of nestin-GFP murine lungs administrated with control or nestin siRNA. Scale bars = 100 μm. (right) Calculation of the GFP/nestin-positive area using Image J (n = 9–13 per group). B Effect of in vivo nestin gene silencing on Notch3 expressing cells (n = 4 per group). C Effect of in vivo nestin gene silencing on mesenchymal-related mRNA expression in the lung (n = 6 per group). D Effect of in vivo nestin gene silencing on αSMA and Col1 expression in lung endothelial cells (n = 6 per group)

Journal: Cell Communication and Signaling : CCS

Article Title: Nestin and Notch3 collaboratively regulate angiogenesis, collagen production, and endothelial–mesenchymal transition in lung endothelial cells

doi: 10.1186/s12964-023-01099-z

Figure Lengend Snippet: Effect of nestin gene silencing in vivo. A (left) GFP immunostaining of nestin-GFP murine lungs administrated with control or nestin siRNA. Scale bars = 100 μm. (right) Calculation of the GFP/nestin-positive area using Image J (n = 9–13 per group). B Effect of in vivo nestin gene silencing on Notch3 expressing cells (n = 4 per group). C Effect of in vivo nestin gene silencing on mesenchymal-related mRNA expression in the lung (n = 6 per group). D Effect of in vivo nestin gene silencing on αSMA and Col1 expression in lung endothelial cells (n = 6 per group)

Article Snippet: The antibodies used by flowcytometry and cell sorting were: CD45-PB (clone 30-F11, BioLegend), EpCAM-PE/Cy7 (clone G8.8, BioLegend), CD140a (PDGFRα)-APC (clone APA-5, BioLegend), CD140b (PDGFRβ)-APC (clone ABP5, Miltenyi Biotec, Bergisch Gladbach, Germany), CD31-APC (clone MEC13-3, BioLegend), CD31-PE/Cy7 (clone 390, BioLegend), VEGFR2-APC (clone Avas12, BioLegend), CD13-PE (clone R3-242, BD Biosciences, San Jose, CA, USA), Notch3-Alexa Fluor 647 (clone HMN3-133, BioLegend), αSMA-eFluor660 (clone 1A4, Thermo Fisher Scientific), mouse IgG2a κ Isotype Control-eFluor 660 (clone eBM2a, Thermo Fisher Scientific), Collagen Type I-FITC (600–401-103–0.1, Rockland Immunochemicals, Pottstown, PA, USA), rabbit IgG Isotype Control-FITC (bs-0295P-FITC, Bioss Antibodies, Woburn, MA, USA), CD105 (TGFβR)-APC (clone MJ7/18, BioLegend), nestin-Alexa Fluor 647 (clone Rat-401, shown as antibody A, BioLegend), Alexa Fluor 647 mouse IgG1, κ Isotype Control (clone MOPC-21, BioLegend), nestin-REAfinity (clone REA575, shown as antibody B, Miltenyi Biotec), REA Control Antibody (I), human IgG1, and REAfinity (clone REA293, Miltenyi Biotec).

Techniques: In Vivo, Immunostaining, Control, Expressing

Association between nestin-expressing cells and collagen production in bleomycin-induced pulmonary fibrosis. A Nes mRNA expression in murine lungs obtained at 2 d after administration with phosphate buffered saline (PBS, control group) or bleomycin (BLM, n = 4 per group). B Changes in CD45 − /nestin-expressing cells during BLM-induced pulmonary fibrosis (n = 4–5 per group). C (left) GFP immunostaining of nestin-GFP murine lungs obtained at 14 d after administration with PBS or BLM. Scale bar = 100 μm. (right) Calculation of the GFP/nestin-positive area using Image J (n = 8 per group). D Flowcytometric analysis of the proportions of nestin-expressing cells in murine lungs 14 d after PBS or BLM treatment using anti-nestin antibody A and B. E The proportions of Notch3 and/or CD13-positive cells in the lungs obtained at 21 d after administration with PBS or BLM (n = 4 per group). F Correlations between the amount of hydroxyproline (indicating collagen content) and the number of CD45 − /nestin-expressing cells in nestin-GFP murine lungs obtained at 7, 14, and 21 d after administration with BLM (n = 4–8 per group)

Journal: Cell Communication and Signaling : CCS

Article Title: Nestin and Notch3 collaboratively regulate angiogenesis, collagen production, and endothelial–mesenchymal transition in lung endothelial cells

doi: 10.1186/s12964-023-01099-z

Figure Lengend Snippet: Association between nestin-expressing cells and collagen production in bleomycin-induced pulmonary fibrosis. A Nes mRNA expression in murine lungs obtained at 2 d after administration with phosphate buffered saline (PBS, control group) or bleomycin (BLM, n = 4 per group). B Changes in CD45 − /nestin-expressing cells during BLM-induced pulmonary fibrosis (n = 4–5 per group). C (left) GFP immunostaining of nestin-GFP murine lungs obtained at 14 d after administration with PBS or BLM. Scale bar = 100 μm. (right) Calculation of the GFP/nestin-positive area using Image J (n = 8 per group). D Flowcytometric analysis of the proportions of nestin-expressing cells in murine lungs 14 d after PBS or BLM treatment using anti-nestin antibody A and B. E The proportions of Notch3 and/or CD13-positive cells in the lungs obtained at 21 d after administration with PBS or BLM (n = 4 per group). F Correlations between the amount of hydroxyproline (indicating collagen content) and the number of CD45 − /nestin-expressing cells in nestin-GFP murine lungs obtained at 7, 14, and 21 d after administration with BLM (n = 4–8 per group)

Article Snippet: The antibodies used by flowcytometry and cell sorting were: CD45-PB (clone 30-F11, BioLegend), EpCAM-PE/Cy7 (clone G8.8, BioLegend), CD140a (PDGFRα)-APC (clone APA-5, BioLegend), CD140b (PDGFRβ)-APC (clone ABP5, Miltenyi Biotec, Bergisch Gladbach, Germany), CD31-APC (clone MEC13-3, BioLegend), CD31-PE/Cy7 (clone 390, BioLegend), VEGFR2-APC (clone Avas12, BioLegend), CD13-PE (clone R3-242, BD Biosciences, San Jose, CA, USA), Notch3-Alexa Fluor 647 (clone HMN3-133, BioLegend), αSMA-eFluor660 (clone 1A4, Thermo Fisher Scientific), mouse IgG2a κ Isotype Control-eFluor 660 (clone eBM2a, Thermo Fisher Scientific), Collagen Type I-FITC (600–401-103–0.1, Rockland Immunochemicals, Pottstown, PA, USA), rabbit IgG Isotype Control-FITC (bs-0295P-FITC, Bioss Antibodies, Woburn, MA, USA), CD105 (TGFβR)-APC (clone MJ7/18, BioLegend), nestin-Alexa Fluor 647 (clone Rat-401, shown as antibody A, BioLegend), Alexa Fluor 647 mouse IgG1, κ Isotype Control (clone MOPC-21, BioLegend), nestin-REAfinity (clone REA575, shown as antibody B, Miltenyi Biotec), REA Control Antibody (I), human IgG1, and REAfinity (clone REA293, Miltenyi Biotec).

Techniques: Expressing, Saline, Control, Immunostaining

Figure 1. Nestin expression (red arrows) in blood vessels in NBTLs [(A) x300], DCIS [(B) x300] and IDC [(C) x300]. Nestin expression in myoepithelial cells is also observed [(A and B) green arrows]. Nestin [(D) x250], CD34 [(E) x250] and CD31 [(F) x250)] expression in blood vessels (red arrows, serial sections).

Journal: International journal of oncology

Article Title: Nestin-positive microvessel density is an independent prognostic factor in breast cancer.

doi: 10.3892/ijo.2017.4057

Figure Lengend Snippet: Figure 1. Nestin expression (red arrows) in blood vessels in NBTLs [(A) x300], DCIS [(B) x300] and IDC [(C) x300]. Nestin expression in myoepithelial cells is also observed [(A and B) green arrows]. Nestin [(D) x250], CD34 [(E) x250] and CD31 [(F) x250)] expression in blood vessels (red arrows, serial sections).

Article Snippet: Immunohistochemical reactions were performed on 4-μm thick paraffin sections using Autostainer Link48 (Dako, Glostrup, Denmark) with a panel of mouse-anti human monoclonal antibodies against: nestin (dilution 1:100, OBT1610, Bio-Rad, Hercules, CA, ISA), CD31 (ready-to-use, IR610, Dako), CD34 (ready-to-use, IR632, Dako) estrogen receptor (ER), clone 1D5 (ready-to-use, IR654; Dako) and progesterone receptor (PR) clone 636 (readyto-use, IR068; Dako).

Techniques: Expressing

SSCs (p2-3) expressed stem cell markers and lacked neural crest markers. ( A ) SSCs derived from female donor 2 expressed CD164, Nestin, CD133, and SOX9 and lacked the expression of SLUG and p75. ( B ) Male donor 14-derived SSCs expressed CD164, Nestin, CD133, and SOX9 but did not express SLUG or p75. ( C ) Semiquantitative analysis showed high Nestin (65.6–89.4%), CD133 (43.5–96.3%), CD164 (82.7–91.0%), and SOX9 (98.1–99.3%) expression in three female SSC populations but lacking p75 (0.0–0.0%) and SLUG (1.1–2.5%) expression. ( D ) Semiquantitative analysis of three male SSC populations unraveled high levels of Nestin (55.4–88.2%), CD133 (50.9–78.8%), CD164 (62.8–75.8%), and SOX9 (97.1–99.0%), but p75 (0.0–0.0%) and SLUG (1.1–3.2%) expressions were deficient.

Journal: Cells

Article Title: Analyzing Sex-Specific Dimorphism in Human Skeletal Stem Cells

doi: 10.3390/cells12232683

Figure Lengend Snippet: SSCs (p2-3) expressed stem cell markers and lacked neural crest markers. ( A ) SSCs derived from female donor 2 expressed CD164, Nestin, CD133, and SOX9 and lacked the expression of SLUG and p75. ( B ) Male donor 14-derived SSCs expressed CD164, Nestin, CD133, and SOX9 but did not express SLUG or p75. ( C ) Semiquantitative analysis showed high Nestin (65.6–89.4%), CD133 (43.5–96.3%), CD164 (82.7–91.0%), and SOX9 (98.1–99.3%) expression in three female SSC populations but lacking p75 (0.0–0.0%) and SLUG (1.1–2.5%) expression. ( D ) Semiquantitative analysis of three male SSC populations unraveled high levels of Nestin (55.4–88.2%), CD133 (50.9–78.8%), CD164 (62.8–75.8%), and SOX9 (97.1–99.0%), but p75 (0.0–0.0%) and SLUG (1.1–3.2%) expressions were deficient.

Article Snippet: Primary antibodies against p65 (rabbit, 1:400, Cell Signaling Technology, Danvers, MA, USA, mAb#8242), Nestin (mouse,1:200, Merck Millipore, Burlington, MA, USA, MAB353), CD164 (mouse, 1:100, Santa Cruz Biotechnology, Dallas, TX, USA, sc-271179), CD133 (rabbit, 1:100, Novus Biologicals, Centennial, CO, USA, NB120-16518,), SLUG (rabbit, 1:400, Cell Signaling Technology, C1967), SOX9 (rabbit,1:400, Invitrogen, Waltham, MA, USA, #702016,), p75 (mouse, 1:100, Sigma Aldrich, N5408), and β-actin (rabbit, 1:200, Cell Signaling Technology, #4970) were diluted in 1× PBS and incubated for 1 h at room temperature.

Techniques: Derivative Assay, Expressing